Project title: Virus-induced chicken hepatoma – a model for investigations on cancerogenesis and antineoplastic activities of metal compounds.

Summary
The LSCC-SF(Mc29) permanent cell line established from a transplantable chicken nepatoma induced by the myelocytomatosis virus Mc29, was cloned and four sublines were obtained – Е7, Е10, D6E10, G9B4. It was found in our previous investigations (Grant CC 1/2000) that these cells differed significantly in morphology, karyotype, proviral load and tumour growth in vivo. The aim of the project presented here is to study comparatively the expression of some tumour associated antigen/s/ (TAA/s/) and tumour suppressor genes (p53) in the experimental model. Hybridoma cells will be developed and monoclonal antibodies (mAbs) obtained will be characterized and used for identifying the TAA/s/ in hepatoma cells. P53 will be evaluated by immunological (usim\ng commercial antibodies) and molecular techniques (PCR, sequencing). Some other avian cells transformed by the myelocytomatosis viruses Mc29 and Mc31 will be included in some of the experiments for comparative investigations.
Cytotoxic and antiproliferative activities of newly synthesyzed metalloporfirins and complexes of Cu(II), Co(II), Zn(II), Ni(II), V(IV), Pt(II), Pd(II) and Ru(II) complexes with different ligands (Schiff and Mannich bases and their derivatives, biguanides, aminoacids) on LSCC-SF(Mc29) cells will be compared to that of some of tumour and nontumour cells from chicken, mouse, rat and human origin. In order to study better the biological activities of metal complexes their antimicrobial effects in vitro will be also studied.

Key words: transplantable chicken nepatoma induced by the myelocytomatosis virus Mc29, tumour associated antigens, monoclonal antibodies, protein p53, cytotoxic/antiproliferative activity, metal compounds/metalloporfirins, cell lines, antimicrobial activity.